Finally, place about 15 glass beads in the third respirometer. This graph shows the effect of temperature on the respiration of peas when put in a water surrounding at 22 degrees celsius. But as Dave Hall said in an earlier post, you really need to make up a fresh solution rather than keeping it year to year. Remove these peas and place them on a paper towel. Ask the home economics teacher for some if you don't have any around the house; a little bit goes a long way. We repeated this set up for three experiments to test the rate of respiration in different temperature conditions.
Hill, Norman High School, Norman, Oklahoma. I have used or known people to use pinto beans, kidney beans, and even lima beans from the store. The graduated cylinder was filled for a third time to 50 milliliters. In fact it is so easy that it makes one wonder why we would even bother doing the lab. This experiment is simple, but elegant and probably teaches our students much about how to correctly set up a controlled experiment. If you don't already have them, try to get them. The vertical axis represents the volume, in milliliters, of water that was drawn into the pipet as a result of intake of oxygen by the peas while they were carrying out respiration.
Trends in the data for germinating peas shows a significant increment difference as time continues on. Add ice to attain the 10 degree C bath. Why are the readings corrected using the glass bead values? Cell Respiration Lab Report Abstract The introduction is where you int roduce the concept of how to do the cellular respiration lab. The paper towels should be wet. Take readings of the volume of water in each pipet every 5 minutes for 20 minutes. It is my personal favorite. Remove these beads and place them on a paper towel.
We found that the germinating peas were the most successful and when we placed our data into our graphs we could easily tell how successful the germinating peas were to the others in oxygen consumption total and increase in time. Why do the glass beads seem to be using oxygen? Methods Summary Chart: Independent Variable: The amount of pea juice in the vials in the germinated vial determined the amount of glass beads that needed to be added to the non-germinating vial. I put them in a large beaker and cover them with water—repeatedly for two days. Cell respiration did occur in the peas. The graduated cylinder was again filled with 50 milliliters of water and 25 non-germinating peas were placed in the water, and then in order to maintain the same volume of the germinating peas, glass beads were added until the non-germinating peas and glass beads combined had the same volume as the 25 germinating peas. Wait five minutes before proceeding.
Experiment 3 This table shows the results of the experiment carried out when the respirometers were submerged in water at 9 degrees celsius. It can be inferred from this information that a higher temperature environment creates a more suitable environment for peas to carry out cellular respiration. Make sure the vials do not fi ll up with water. I count out the needed number of peas for the students. So much for only having one independent variable. Write a hypothesis using the same experimental design to compare the rates of res- piration in a mouse at both room temperature 24° C and at 10° C. Start to record the temperature of the thermometer every minute for 15 minutes.
Measuring Respiration of Germinating and Non-germinating Peas By: Krunal Patel Introduction Living cells require transfusions of energy from outside sources to perform their many tasks — for example, assembling polymers, pumping substances across membranes, moving, and reproducing Campbell, and Reece 162. Compare the rate of oxygen consumption at 10°they different? Next, add enough glass beads to equal the volume of the germinating peas. This allows them to have high oxygen consumption rates or respiration rates in this experiment. There are three ways to measure the rate of cellular respiration. Drop in 25 germinating peas and determine the amount of water that was displaced. In fact we test it at different temperatures and with different sugars sucrose, glucose, and lactose.
However, it's always nice when some young student with 17-year-old eyes has to ask me to find the meniscus for them!! Wow, did that simplify things and work better! All three respirometers successfully drew in water through the pipet as a result of the respiration of the peas. I have found it is necessary to reapply the silicon cement on a yearly basis: off with the old, on with the new. The rate of respiration in germinating peas was compared to the rate of the non-geminating peas. There are several factors that may cause a pressure change other than pea respiration. Introduction In this lab we will measure oxygen consumption during respiration as the change in gas volume in respirometers containing germinating or nongerminating peas. This is why we had a corrected difference for the glass beads. I played around with it, and found that if I put a handful of peas in, the results were more dramatic.
The next step in respiration is the Krebs cycle. In the first respirometer, place germinating peas. In plant cells, it occurs after germination begins. Hands should be kept out of the water bath after the experiment has started. The graph shows how both germinating peas at high or low temperature were much more successful in oxygen consumption and high rate. For this we are comparing both the germinating and the nongerminating peas at two different temperatures.
Determine the volume of germinated peas. The vial with glass beads only will permit detection of any changes in volume due to atmospheric pressure changes or temperature changes. The experiment required six respirometers to be set up as follows. The students were at first very disappointed, but came to see how it could help them write a better lab design on a free-response question. Our hypothesis was correct that germinating peas consumed more oxygen than the non germinating peas and beads in warmer temperatures than in cooler temperatures. Germinating and Non- germ slope: From the slope of the lines, determine the rate of oxygen consumption at 10°room temperature for the germinating and non-germinating pea seeds. But, it is an excellent lab for getting students to look at the data and figure out what might be going on—such as what sorts of things did we not control or not do a good job of controlling, leakage, inadequate time to come to constant experimental temperature, expansion of atmospheric air in respirometer, and so on.
But I still opt to use the gas pressure sensor. Organisms release this stored energy by the breakdown of glucose. The hypothesis of this lab states that if the peas are germinated then the rate of cellular respiration will be higher in both room temperature and cold temperature. If the respirometers leak during the experiment, you will have to start over. To add to this, even the germinating peas at cold temperature is still greater than the non-germinating peas at high temperature.